6FJC

Human KIBRA C2 domain mutant C771A in complex with phosphatidylinositol 3,4,5-trisphosphate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	CCD
Collection date	2014-09-04
Detector	RIGAKU SATURN 944+
Wavelength(s)	1.54
Spacegroup name	P 61 2 2
Unit cell lengths	83.404, 83.404, 208.254
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	72.230 - 2.598
R-factor	0.1952
Rwork	0.192
R-free	0.26390
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2z0u
RMSD bond length	0.008
RMSD bond angle	1.133
Data reduction software	d*TREK
Data scaling software	d*TREK
Phasing software	BALBES
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	72.230	2.690
High resolution limit [Å]	2.598	2.598
Rmerge	0.167	0.621
Rmeas	0.174	0.662
Number of reflections	13926
<I/σ(I)>	9.7	2.3
Completeness [%]	100.0	100
Redundancy	12.09	8.34

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	0.1M Tris pH 8.0, 1.5M (NH4)2SO4