6FJ3
High resolution crystal structure of parathyroid hormone 1 receptor in complex with a peptide agonist.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-09-04 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.000 |
Spacegroup name | P 1 |
Unit cell lengths | 44.115, 52.864, 111.874 |
Unit cell angles | 80.63, 83.76, 79.16 |
Refinement procedure
Resolution | 49.386 - 2.500 |
R-factor | 0.211 |
Rwork | 0.209 |
R-free | 0.24830 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5ee7 3c4m |
RMSD bond length | 0.002 |
RMSD bond angle | 0.506 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.390 | 2.600 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.153 | 1.662 |
Rpim | 0.103 | 1.214 |
Number of reflections | 33759 | 3825 |
<I/σ(I)> | 5.2 | 0.9 |
Completeness [%] | 99.8 | 99.3 |
Redundancy | 5.3 | 4.7 |
CC(1/2) | 0.994 | 0.501 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 6 | 293 | 0.1 M sodium citrate pH 6.0, 0.3 M sodium acetate, 31% PEG400, 20 uM E-PTH(1-34) |