6FFA
FMDV Leader protease bound to substrate ISG15
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-10-17 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.92819 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 114.188, 40.352, 58.052 |
| Unit cell angles | 90.00, 91.81, 90.00 |
Refinement procedure
| Resolution | 58.023 - 1.500 |
| R-factor | 0.1662 |
| Rwork | 0.165 |
| R-free | 0.18630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qol |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.339 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.023 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.044 | 0.489 |
| Number of reflections | 42227 | 2043 |
| <I/σ(I)> | 13.4 | 2.2 |
| Completeness [%] | 99.2 | 98.4 |
| Redundancy | 4.2 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 2 M Ammonium Sulfate 0.2 M Sodium potassium tartrate tetrahydrate 0.1 M Sodium citrate tribasic (pH 5.6) |
