6FF4
human Bact spliceosome core structure
EM specimen
| Vitrification | Yes |
| Instrument | LEICA EM GP |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 0.001 |
| Detector | FEI FALCON III (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.4 |
| Number of Particle | 308000 |
| Software | RELION,PHENIX,Gctf,UCSF Chimera,EMAN2 |
| CTF correction | PHASE FLIPPING ONLY |
