6FEK
Oncogenic point mutation of RET receptor tyrosine kinase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-03-29 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 50.901, 50.901, 242.515 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.934 - 2.300 |
| R-factor | 0.2029 |
| Rwork | 0.201 |
| R-free | 0.24410 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.582 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.500 | 2.190 |
| High resolution limit [Å] | 2.080 | 2.080 |
| Rpim | 0.046 | 0.340 |
| Number of reflections | 27003 | 1364 |
| <I/σ(I)> | 12.8 | 2 |
| Completeness [%] | 98.5 | 97 |
| Redundancy | 24.5 | 25.5 |
| CC(1/2) | 0.998 | 0.641 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.4 | 289 | 3.4 M sodium formate, and 0.1 M sodium acetate pH 4.4 |
