6FDF

Crystal structure of S. pombe Dnmt2 methyltransferase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-09-12
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1.033
Spacegroup name	C 1 2 1
Unit cell lengths	153.817, 114.781, 113.759
Unit cell angles	90.00, 131.05, 90.00

Refinement procedure

Resolution	40.795 - 1.697
R-factor	0.1742
Rwork	0.174
R-free	0.19480
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.795	40.795	1.800
High resolution limit [Å]	1.697	30.000	1.700
Rmerge	0.030	0.029	0.509
Rmeas	0.035	0.039	0.598
Number of reflections	162497	16	25533
<I/σ(I)>	20.63	32.9	2.69
Completeness [%]	99.0	41	99.3
Redundancy	3.492	2.188	3.574
CC(1/2)	0.999	0.996	0.813

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277.15	50 mM MES pH 6.5 1 % w/v PEG 4000 4 mM MgCl2