6FB9
Crystal Structure of the I-CreI Homing Endonuclease D75N variant in complex with an altered version of its target DNA at 5NNN region in the presence of Manganese
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-07-02 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.8 |
| Spacegroup name | P 43 |
| Unit cell lengths | 79.729, 79.729, 126.250 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.049 - 2.950 |
| R-factor | 0.1712 |
| Rwork | 0.168 |
| R-free | 0.20330 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1g9z |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.294 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.490 | 3.110 |
| High resolution limit [Å] | 2.950 | 2.950 |
| Rmerge | 0.080 | 0.560 |
| Number of reflections | 32279 | |
| <I/σ(I)> | 12.4 | |
| Completeness [%] | 98.6 | |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.1 M calcium acetate, 0.1 M sodium acetate pH 4.6-5.4, 33-40% (v/v) 1,2-propanediol |
