6FAE
The Sec7 domain of IQSEC2 (Brag1) in complex with the small GTPase Arf1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-09-17 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.9159 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.995, 92.744, 66.895 |
| Unit cell angles | 90.00, 105.53, 90.00 |
Refinement procedure
| Resolution | 49.134 - 2.350 |
| R-factor | 0.2426 |
| Rwork | 0.240 |
| R-free | 0.28510 |
| RMSD bond length | 0.001 |
| RMSD bond angle | 0.392 |
| Data reduction software | DIALS |
| Data scaling software | DIALS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.140 | 2.410 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Number of reflections | 25045 | |
| <I/σ(I)> | 8.9 | |
| Completeness [%] | 99.9 | |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 0.1 M tris 16% PEG 8K 0.1M Potassium chloride |
