6F58
Crystal structure of human Brachyury (T) in complex with DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-08-04 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.92 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 74.978, 37.109, 110.796 |
Unit cell angles | 90.00, 103.99, 90.00 |
Refinement procedure
Resolution | 29.649 - 2.253 |
R-factor | 0.2445 |
Rwork | 0.242 |
R-free | 0.28770 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1xbr |
RMSD bond length | 0.004 |
RMSD bond angle | 0.682 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.650 | 2.330 |
High resolution limit [Å] | 2.250 | 2.250 |
Rmerge | 0.123 | 0.689 |
Rmeas | 0.940 | |
Rpim | 0.079 | 0.501 |
Number of reflections | 27866 | 2466 |
<I/σ(I)> | 6.1 | 1.5 |
Completeness [%] | 97.6 | 94.1 |
Redundancy | 3.4 | 3.4 |
CC(1/2) | 0.992 | 0.653 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 277 | 40% PEG300 -- 0.1M citrate pH 4.2 |