6F3M
Crystal structure of S-adenosyl-L-homocysteine hydrolase from Pseudomonas aeruginosa complexed with adenosine, K+ and Zn2+ cations
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-11-21 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.9184 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 175.976, 104.145, 107.634 |
| Unit cell angles | 90.00, 100.71, 90.00 |
Refinement procedure
| Resolution | 48.700 - 1.600 |
| R-factor | 0.1512 |
| Rwork | 0.151 |
| R-free | 0.17421 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6f3p |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.580 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.690 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.061 | |
| Number of reflections | 244909 | |
| <I/σ(I)> | 14.9 | |
| Completeness [%] | 97.8 | |
| Redundancy | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 292 | 50 mM KH2PO4, 20 % (W/V) PEG8000, 2 mM adenosine |
