6F09
Binary complex of 14-3-3 zeta with ubiquitin specific protease 8 (USP8) pSer718 peptide
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-09-07 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.000 |
Spacegroup name | P 1 |
Unit cell lengths | 60.379, 64.890, 71.519 |
Unit cell angles | 99.71, 114.22, 108.79 |
Refinement procedure
Resolution | 61.137 - 1.594 |
R-factor | 0.1822 |
Rwork | 0.180 |
R-free | 0.22470 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4hkc |
RMSD bond length | 0.010 |
RMSD bond angle | 1.011 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 61.137 | 1.620 |
High resolution limit [Å] | 1.594 | 1.594 |
Rmerge | 0.062 | |
Rmeas | 0.088 | |
Rpim | 0.062 | |
Number of reflections | 107299 | |
<I/σ(I)> | 1.84 | |
Completeness [%] | 91.5 | |
Redundancy | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298.15 | 0.1 M phosphate citrate pH 4.4; 40% PEG 300 |