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6F09

Binary complex of 14-3-3 zeta with ubiquitin specific protease 8 (USP8) pSer718 peptide

Experimental procedure
Experimental methodMAD
Source typeSYNCHROTRON
Source detailsSLS BEAMLINE X06DA
Synchrotron siteSLS
BeamlineX06DA
Temperature [K]100
Detector technologyPIXEL
Collection date2017-09-07
DetectorDECTRIS PILATUS 6M
Wavelength(s)1.000
Spacegroup nameP 1
Unit cell lengths60.379, 64.890, 71.519
Unit cell angles99.71, 114.22, 108.79
Refinement procedure
Resolution61.137 - 1.594
R-factor0.1822
Rwork0.180
R-free0.22470
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)4hkc
RMSD bond length0.010
RMSD bond angle1.011
Data reduction softwareiMOSFLM
Data scaling softwareAimless
Phasing softwareMOLREP
Refinement softwarePHENIX ((1.12_2829: ???))
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]61.1371.620
High resolution limit [Å]1.5941.594
Rmerge0.062
Rmeas0.088
Rpim0.062
Number of reflections107299
<I/σ(I)>1.84
Completeness [%]91.5
Redundancy3.3
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP298.150.1 M phosphate citrate pH 4.4; 40% PEG 300

217705

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