6F00
Crystal Structure of human tRNA dihydrouridine synthase (20) dsRBD E423A mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 2 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-06-29 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.976254 |
Spacegroup name | P 1 |
Unit cell lengths | 52.250, 52.210, 52.310 |
Unit cell angles | 107.69, 107.92, 107.65 |
Refinement procedure
Resolution | 44.700 - 2.163 |
R-factor | 0.1791 |
Rwork | 0.176 |
R-free | 0.22770 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4wft |
RMSD bond length | 0.013 |
RMSD bond angle | 1.102 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.700 | 2.240 |
High resolution limit [Å] | 2.163 | 2.163 |
Rmerge | 0.072 | 0.641 |
Number of reflections | 41560 | 2174 |
<I/σ(I)> | 7.58 | 1.3 |
Completeness [%] | 96.0 | 92.16 |
Redundancy | 2 | 2 |
CC(1/2) | 0.995 | 0.481 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 292 | 1.6 M ammonium sulfate 100 mM sodium chloride 100 mM HEPES |