6EYN
Structure of the 8D6 (anti-IgE) Fab
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-10-01 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9795 |
Spacegroup name | P 43 |
Unit cell lengths | 157.957, 157.957, 77.503 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.553 - 2.400 |
R-factor | 0.1828 |
Rwork | 0.181 |
R-free | 0.21210 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1i7z |
RMSD bond length | 0.003 |
RMSD bond angle | 0.643 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 70.640 | 2.450 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.123 | 1.470 |
Rpim | 0.049 | 0.581 |
Number of reflections | 74039 | 4617 |
<I/σ(I)> | 10.5 | 1.9 |
Completeness [%] | 98.8 | 99.9 |
Redundancy | 6.2 | 6.3 |
CC(1/2) | 0.996 | 0.643 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 0.1M ADA (N-(2-acetamido) iminodiacetic acid) pH6.5 and 12% (w/v) PEG 4000 |