6EY7
Human cytomegalovirus terminase nuclease domain, Mn soaked, inhibitor bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-07-18 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9194, 1.240 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 81.650, 87.650, 186.580 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.870 - 2.900 |
| R-factor | 0.176 |
| Rwork | 0.175 |
| R-free | 0.19800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3n4p |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.090 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.950 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmeas | 0.080 | 1.030 |
| Number of reflections | 30097 | 1502 |
| <I/σ(I)> | 14.4 | 1.8 |
| Completeness [%] | 98.8 | 99.7 |
| Redundancy | 5.2 | 5.6 |
| CC(1/2) | 0.998 | 0.654 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 10% Mes 1M pH 6.5, 6% PEG8000 and 150 mM calcium acetate |
