6EPA

Structure of dNCS-1 bound to the NCS-1/Ric8a protein/protein interaction regulator IGS-1.76

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-03-10
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979256
Spacegroup name	P 21 21 21
Unit cell lengths	54.843, 55.456, 61.259
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.112 - 1.820
R-factor	0.1905
Rwork	0.189
R-free	0.23190
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4by4
RMSD bond length	0.008
RMSD bond angle	0.554
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.11_2567: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.112	1.860
High resolution limit [Å]	1.820	1.820
Rpim	0.029	0.825
Number of reflections	116619	1008
<I/σ(I)>	14.4	0.9
Completeness [%]	100.0	100
Redundancy	6.7	4.3
CC(1/2)	0.999	0.389

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293.15	0.1 M Hepes pH 7.5 and 25% w/v PEG 2000 MME