6EKK
Crystal structure of GEF domain of DENND 1A in complex with Rab GTPase Rab35-GDP bound state.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-02-13 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.9686 |
Spacegroup name | P 1 |
Unit cell lengths | 55.700, 67.990, 101.710 |
Unit cell angles | 80.50, 76.80, 67.06 |
Refinement procedure
Resolution | 50.452 - 1.820 |
R-factor | 0.1954 |
Rwork | 0.194 |
R-free | 0.23230 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3tw8 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.104 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | MOLREP |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.452 | 1.870 |
High resolution limit [Å] | 1.820 | 1.820 |
Number of reflections | 115286 | |
<I/σ(I)> | 8.5 | |
Completeness [%] | 95.7 | |
Redundancy | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 277 | 25%PEG3350, 0.2M AMS, 0.1M Bis-Tris pH 5.5 |