6EJW
Tryptophan Repressor TrpR from E.coli wildtype with Indole-3-acetic acid as ligand
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-12-13 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.999900 |
Spacegroup name | P 43 |
Unit cell lengths | 81.310, 81.310, 72.000 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.928 - 1.990 |
R-factor | 0.1969 |
Rwork | 0.195 |
R-free | 0.24290 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1tro |
RMSD bond length | 0.008 |
RMSD bond angle | 0.902 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER (2.5.6) |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 44.928 | 44.928 | 2.040 |
High resolution limit [Å] | 1.990 | 8.900 | 1.990 |
Rmerge | 0.092 | 0.025 | 2.071 |
Rmeas | 0.101 | 0.027 | 2.270 |
Number of reflections | 32112 | 380 | 2238 |
<I/σ(I)> | 11.25 | 36.84 | 0.95 |
Completeness [%] | 99.4 | 99 | 93.7 |
Redundancy | 6.494 | 6.547 | 5.75 |
CC(1/2) | 0.999 | 1.000 | 0.432 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 0.1 M Bis-Tris pH 5.5 2 M Ammonium sulfate |