1TRO

CRYSTAL STRUCTURE OF TRP REPRESSOR OPERATOR COMPLEX AT ATOMIC RESOLUTION

Summary for 1TRO

• Entry DOI: 10.2210/pdb1tro/pdb
• Descriptor: DNA (5'-D(*TP*GP*TP*AP*CP*TP*AP*GP*TP*TP*AP*AP*CP*TP*AP*GP*T P*AP*C)-3'), PROTEIN (TRP REPRESSOR), TRYPTOPHAN, ... (4 entities in total)
• Functional Keywords: protein-dna complex, double helix, transcription-dna complex, transcription/dna
• Biological source: Escherichia coli str. K12 substr.
• Total number of polymer chains: 8
• Total formula weight: 73576.54

Authors

Otwinowski, Z.,Schevitz, R.W.,Zhang, R.-G.,Lawson, C.L.,Joachimiak, A.,Marmorstein, R.,Luisi, B.F.,Sigler, P.B. (deposition date: 1992-08-30, release date: 1994-01-31, Last modification date: 2024-02-14)

Primary citation

Otwinowski, Z.,Schevitz, R.W.,Zhang, R.G.,Lawson, C.L.,Joachimiak, A.,Marmorstein, R.Q.,Luisi, B.F.,Sigler, P.B.
Crystal structure of trp repressor/operator complex at atomic resolution.
Nature, 335:321-329, 1988

PubMed Abstract: The crystal structure of the trp repressor/operator complex shows an extensive contact surface, including 24 direct and 6 solvent-mediated hydrogen bonds to the phosphate groups of the DNA. There are no direct hydrogen bonds or non-polar contacts to the bases that can explain the repressor's specificity for the operator sequence. Rather, the sequence seems to be recognized indirectly through its effects on the geometry of the phosphate backbone, which in turn permits the formation of a stable interface. Water-mediated polar contacts to the bases also appear to contribute part of the specificity.

PubMed: 3419502
DOI: 10.1038/335321a0

Experimental method

X-RAY DIFFRACTION (1.9 Å)