6EIY

Crystal structure of KDM5B in complex with KDOPZ000034a.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-03-10
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97950
Spacegroup name	P 65 2 2
Unit cell lengths	142.380, 142.380, 152.170
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	51.983 - 2.150
R-factor	0.1876
Rwork	0.186
R-free	0.21540
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5a1f
Data reduction software	xia2
Data scaling software	xia2
Phasing software	MOLREP
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	51.983	2.210
High resolution limit [Å]	2.150	2.150
Rmerge	0.116	2.524
Number of reflections	49958	3615
<I/σ(I)>	18.7	1.3
Completeness [%]	100.0	100
Redundancy	19.6	19
CC(1/2)		0.586

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	0.1M HEPES pH 7.5, 0.8M Pottassium Phosphate-dibasic, 0.8M Sodium Phosphate monobasic