6EIM

Human STK10 bound to GW683134A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-11-25
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	49.468, 99.812, 125.839
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.806 - 1.430
R-factor	0.1554
Rwork	0.154
R-free	0.17360
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2j7t
RMSD bond length	0.004
RMSD bond angle	0.676
Data reduction software	XDS
Data scaling software	Aimless (0.5.29)
Phasing software	PHASER (2.6.1)
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.390	46.390	1.480
High resolution limit [Å]	1.430	5.540	1.430
Rmerge	0.053	0.034	0.839
Rmeas	0.061	0.039	0.958
Rpim	0.028	0.018	0.450
Total number of observations	449512
Number of reflections	110558	1886	10616
<I/σ(I)>	12.6
Completeness [%]	96.0	87.9	94.8
Redundancy	4.1	4	3.9
CC(1/2)	0.999	0.998	0.535

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	10% PEG1000, 10% PEG8000