6EHT
Modulation of PCNA sliding surface by p15PAF suggests a suppressive mechanism for cisplatin-induced DNA lesion bypass by pol eta holoenzyme
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-02-07 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 75.993, 42.300, 141.827 |
Unit cell angles | 90.00, 102.70, 90.00 |
Refinement procedure
Resolution | 40.480 - 3.200 |
R-factor | 0.26401 |
Rwork | 0.261 |
R-free | 0.32715 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4d2g |
RMSD bond length | 0.011 |
RMSD bond angle | 1.396 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0222) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.120 | 3.370 |
High resolution limit [Å] | 3.200 | 3.200 |
Rmerge | 0.105 | 0.320 |
Number of reflections | 14271 | 2122 |
<I/σ(I)> | 5.7 | 2.2 |
Completeness [%] | 95.3 | 98.8 |
Redundancy | 2.2 | 2.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 10% polyethylene glycol 3350 0.1 M sodium acetate pH 4.5 |