6EHG

complement component C3b in complex with a nanobody

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-05-29
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.98021
Spacegroup name	P 21 21 2
Unit cell lengths	255.310, 64.880, 144.720
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	62.880 - 2.650
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5fo7
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((dev_2614: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	62.880
High resolution limit [Å]	2.650
Number of reflections	70861
<I/σ(I)>	17.42
Completeness [%]	99.8
Redundancy	40.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	12 mM Sodium Citrate pH 8, 28 mM Sodium Citrate pH 6.0, 12.25 % (w/v) PEG 2000 MME, 70 mM Imidazole pH 7, 60 mM Ammonium acetate, 70 mM Tris pH 8.5, 13.5 % (v/v) and (+/-)-2-Methyl-2,4-pentanediol