6EHA
Heme oxygenase 1 in complex with inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-03-10 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.918409 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.411, 54.566, 72.540 |
| Unit cell angles | 90.00, 99.54, 90.00 |
Refinement procedure
| Resolution | 42.850 - 2.000 |
| R-factor | 0.19162 |
| Rwork | 0.189 |
| R-free | 0.23876 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.599 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.850 | 2.071 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.115 | 0.563 |
| Number of reflections | 32213 | |
| <I/σ(I)> | 5.28 | 1.65 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 1.9 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 2.08 M AmSO4 0.9 % (v/v) 1,6-hexanediol 0.1 M HEPES pH 7.5 |
