6EGS
Crystal structure of the GalNAc-T2 F104S mutant in complex with UDP-GalNAc
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-02-12 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.97 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 154.036, 154.036, 109.823 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 154.040 - 2.700 |
R-factor | 0.20669 |
Rwork | 0.206 |
R-free | 0.22104 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4d0t |
RMSD bond length | 0.011 |
RMSD bond angle | 1.529 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 154.040 | 2.850 |
High resolution limit [Å] | 2.700 | 2.700 |
Rpim | 0.064 | 0.720 |
Number of reflections | 70380 | |
<I/σ(I)> | 7.8 | 1.1 |
Completeness [%] | 99.7 | 100 |
Redundancy | 5.6 | 5.5 |
CC(1/2) | 0.995 | 0.371 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | PEG6000, Hepes |