6EEM

Crystal structure of Papaver somniferum tyrosine decarboxylase in complex with L-tyrosine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	80
Detector technology	CCD
Collection date	2014-11-05
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979180
Spacegroup name	P 41 21 2
Unit cell lengths	122.771, 122.771, 166.998
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	77.026 - 2.610
R-factor	0.189602041113
Rwork	0.187
R-free	0.23453
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1js3
RMSD bond length	0.004
RMSD bond angle	0.709
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	167.000	2.750
High resolution limit [Å]	2.610	2.610
Number of reflections	39550	5654
<I/σ(I)>	9.1	1.6
Completeness [%]	100.0	100
Redundancy	8.4	8.5
CC(1/2)	0.985	0.567

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	277	1.2 M ammonium Sulfate 0.1 Bis Tris pH 5.0 and 1% w/v PEG 3350