6EDB
Crystal structure of SRY.hcGAS-21bp dsDNA complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-04-05 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9792 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 90.307, 115.827, 159.077 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.008 - 3.209 |
R-factor | 0.2598 |
Rwork | 0.255 |
R-free | 0.30160 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4k96 |
RMSD bond length | 0.019 |
RMSD bond angle | 0.568 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.008 | 3.320 |
High resolution limit [Å] | 3.200 | 3.200 |
Rpim | 0.051 | 0.338 |
Number of reflections | 12639 | 833 |
<I/σ(I)> | 13.4 | |
Completeness [%] | 60.4 | |
Redundancy | 5.4 | |
CC(1/2) | 0.980 | 0.722 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2 M L-proline, 0.1 M HEPES, pH 7.5, 8% PEG3350 (v/v), 10 mM MgCl2 |