6EAY

Structural Basis for Broad Neutralization of Ebolaviruses by an Antibody Targeting the Glycoprotein Fusion Loop

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2016-11-27
Detector	RAYONIX MX300-HS
Wavelength(s)	1
Spacegroup name	H 3 2
Unit cell lengths	153.429, 153.429, 335.862
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.128 - 3.720
R-factor	0.2604
Rwork	0.259
R-free	0.29660
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5jq3 3q6g
RMSD bond length	0.002
RMSD bond angle	0.570
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3228: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	76.710	4.070
High resolution limit [Å]	3.720	3.720
Rmerge	0.181	0.589
Rpim	0.054	0.176
Number of reflections	14037	1402
<I/σ(I)>	9.9	3.9
Completeness [%]	87.2	88.5
Redundancy	9.8	9.7
CC(1/2)	0.993	0.859

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	298	1M Potassium Sodium Tartrate, 0.1M MES pH 6.0