6EA7
Structure of EBOV GPcl in complex with the pan-ebolavirus mAb ADI-15878
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-04-09 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 152.781, 152.781, 247.685 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 56.080 - 4.250 |
| R-factor | 0.2832 |
| Rwork | 0.280 |
| R-free | 0.32390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5hj3 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.655 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.080 | 4.400 |
| High resolution limit [Å] | 4.250 | 4.250 |
| Number of reflections | 24218 | 4317 |
| <I/σ(I)> | 10.6 | 2.1 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 173.1 | 162.8 |
| CC(1/2) | 1.000 | 0.560 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | 100 mM MgCl2, 100 mM HEPES pH 7.5, and 10% PEG 4000 |
