6E7Y
cryo-EM structure of human TRPML1 with PI45P2
EM specimen
Vitrification | Yes |
EM Data Collection
Microscope | FEI TITAN KRIOS |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 3.57 |
Software | RELION,FREALIX,CTFFIND |
CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |