6E7M
Crystal structure of the holo retinal-bound domain-swapped dimer Q108K:T51D:A28C mutant of human Cellular Retinol Binding Protein II
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-07-20 |
Detector | RAYONIX MX300HE |
Wavelength(s) | 0.9782 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 67.739, 72.067, 112.252 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.358 - 2.700 |
R-factor | 0.2364 |
Rwork | 0.230 |
R-free | 0.29610 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2rct |
RMSD bond length | 0.002 |
RMSD bond angle | 0.490 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11.1_2575)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.360 | 2.800 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmeas | 0.197 | |
Rpim | 0.775 | |
Number of reflections | 15836 | |
<I/σ(I)> | 11.5 | |
Completeness [%] | 100.0 | |
Redundancy | 7.2 | |
CC(1/2) | 0.389 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | PEG 4000, ammonium acetate, sodium acetate |