6E6C

HRAS G13D bound to GppNHp (H13GNP)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	173.15
Detector technology	IMAGE PLATE
Collection date	2014-03-28
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54178
Spacegroup name	C 1 2 1
Unit cell lengths	54.600, 47.753, 57.037
Unit cell angles	90.00, 118.31, 90.00

Refinement procedure

Resolution	25.100 - 1.900
Rwork	0.168
R-free	0.23000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3k8y
Data reduction software	HKL-3000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	1.930
High resolution limit [Å]	1.900	5.150	1.900
Rmerge	0.088	0.058	0.542
Rmeas	0.104	0.069	0.670
Rpim	0.054	0.037	0.388
Number of reflections	9755	527	405
<I/σ(I)>	6.8
Completeness [%]	95.0	98.9	84.4
Redundancy	3.5	3.4	2.5
CC(1/2)		0.994	0.668

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	291.15	128 mM Calcium acetate, 20.8% PEG 3350, 20% stabilization buffer (20 mM HEPES, 50 mM NaCl, 20 mM MgCl2, at pH 7.5), Crystals were grown in 2uL by 2 uL drops of mother liquor to protein (22 mg/mL), Cryoprotectant: 70% glycerol and 30% mother liquor