6E56

Human antibody H2214 in complex with influenza hemagglutinin A/Aichi/2/1968 (X-31) (H3N2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-23
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 21 2 21
Unit cell lengths	77.550, 98.710, 224.870
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.851 - 2.000
R-factor	0.189
Rwork	0.188
R-free	0.21440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB entries 6B0A 4fp8 5y11 4yk4
RMSD bond length	0.004
RMSD bond angle	0.652
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.851	2.072
High resolution limit [Å]	2.000	2.000
Rmerge	0.106	0.121
Number of reflections	117279	66956
<I/σ(I)>	10.67	1.45
Completeness [%]	99.4	99.92
Redundancy	5.6	5.8
CC(1/2)	0.997	0.544

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	291.15	100 mM sodium chloride, 100 mM sodium acetate, pH 5.0, 20% w/v PEG2000 MME