6DW7

SAMHD1 without Catalytic Nucleotides

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-05-25
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.97912
Spacegroup name	P 1 21 1
Unit cell lengths	84.546, 146.372, 98.929
Unit cell angles	90.00, 113.67, 90.00

Refinement procedure

Resolution	50.000 - 2.500
R-factor	0.21427
Rwork	0.212
R-free	0.25044
RMSD bond length	0.011
RMSD bond angle	1.681
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.540
High resolution limit [Å]	2.500	2.500
Rmerge	0.126
Number of reflections	76374	3868
<I/σ(I)>	6.3
Completeness [%]	97.5	98.7
Redundancy	2.9	3
CC(1/2)	0.985	0.427

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH		298	100 MM BIS-TRIS PH 6.7 AND 25% (W/V) PEG1500