6DS6
Crystal structure of p300 ZZ domain in complex with histone H3 peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 4.2.2 |
Synchrotron site | ALS |
Beamline | 4.2.2 |
Temperature [K] | 100 |
Detector technology | CMOS |
Collection date | 2017-04-22 |
Detector | RDI CMOS_8M |
Wavelength(s) | 1.278 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 44.026, 44.026, 85.904 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 21.320 - 1.950 |
R-factor | 0.2117 |
Rwork | 0.207 |
R-free | 0.25320 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 0.922 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.180 | 1.980 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.077 | 0.296 |
Number of reflections | 6576 | 292 |
<I/σ(I)> | 71.4 | |
Completeness [%] | 99.2 | |
Redundancy | 22.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.1 M HEPES and 70% MPD (pH 7.5) |