6DP3
Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with an RNA/DNA Hybrid: Reaction in 10 mM Mg2+ and 75 mM K+ for 40 s at 21 C
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-06-23 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 81.530, 38.108, 61.723 |
Unit cell angles | 90.00, 96.04, 90.00 |
Refinement procedure
Resolution | 19.090 - 1.461 |
R-factor | 0.1721 |
Rwork | 0.171 |
R-free | 0.19800 |
Starting model (for MR) | 1zbl |
RMSD bond length | 0.008 |
RMSD bond angle | 1.085 |
Data reduction software | HKL-2000 |
Data scaling software | Aimless (0.5.31) |
Phasing software | PHENIX |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 19.090 | 19.090 | 1.490 |
High resolution limit [Å] | 1.460 | 8.000 | 1.460 |
Total number of observations | 32665 | ||
Number of reflections | 32665 | 199 | 1508 |
<I/σ(I)> | 6.7 | ||
Completeness [%] | 99.3 | 91 | 94.4 |
Redundancy | 1 | 1 | 1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 294 | 14% PEG3350, 20% glycerol, 200 mM KI, and 25 mM CaCl2 |