6DO3

KLHDC2 ubiquitin ligase in complex with SelK C-end degron

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-12-07
Detector	ADSC QUANTUM 315r
Wavelength(s)	1
Spacegroup name	P 1 21 1
Unit cell lengths	44.816, 87.779, 88.626
Unit cell angles	90.00, 104.49, 90.00

Refinement procedure

Resolution	43.391 - 2.165
R-factor	0.1691
Rwork	0.166
R-free	0.22320
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2vpj
RMSD bond length	0.008
RMSD bond angle	1.160
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.190
High resolution limit [Å]	2.150	5.830	2.150
Rmerge	0.168	0.084	0.598
Rmeas	0.182	0.091	0.679
Rpim	0.069	0.036	0.316
Number of reflections	35125	1817	1759
<I/σ(I)>	6.4
Completeness [%]	99.9	99.8	99.2
Redundancy	6.9	6.7	4.3
CC(1/2)		0.995	0.772

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298.15	0.03M MgCl2*6H2O, 0.03M CaCl2*2H2O, 0.05M Imidazole, 0.05M MES monohydrate, 12.5% v/v MPD, 12.5% PEG1000, 12.5% w/v PEG3350, pH 6.5.