6DLD
Crystal structure of IgLON5/NEGR1 heterodimer
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F1 |
Synchrotron site | CHESS |
Beamline | F1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-12-03 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.98 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 120.341, 305.799, 60.029 |
Unit cell angles | 90.00, 100.78, 90.00 |
Refinement procedure
Resolution | 48.596 - 3.300 |
R-factor | 0.247 |
Rwork | 0.245 |
R-free | 0.28590 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5eo9 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.569 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.600 | 3.500 |
High resolution limit [Å] | 3.300 | 3.300 |
Number of reflections | 31695 | |
<I/σ(I)> | 7.92 | |
Completeness [%] | 98.8 | |
Redundancy | 3.5 | |
CC(1/2) | 0.996 | 0.397 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | MES-NaOH pH6.5, MgSO4 |