6DFX
human diabetogenic TCR T1D3 in complex with DQ8-p8E9E peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-03-22 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.979100 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 111.705, 88.896, 116.777 |
Unit cell angles | 90.00, 104.26, 90.00 |
Refinement procedure
Resolution | 50.010 - 2.030 |
R-factor | 0.18273 |
Rwork | 0.181 |
R-free | 0.21657 |
RMSD bond length | 0.028 |
RMSD bond angle | 2.493 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.200 |
High resolution limit [Å] | 2.030 | 2.030 |
Number of reflections | 142449 | |
<I/σ(I)> | 14.4 | |
Completeness [%] | 98.1 | |
Redundancy | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 278 | 10% PEG 8000, 100mM cacodylate pH6.2, 400mM NaCl |