6DC9
Fab/epitope complex of human chimeric monoclonal antibody h4E6 targeting a phosphorylated tau epitope.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL14-1 |
| Synchrotron site | SSRL |
| Beamline | BL14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-07-18 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 79.212, 65.080, 97.439 |
| Unit cell angles | 90.00, 112.11, 90.00 |
Refinement procedure
| Resolution | 48.692 - 2.999 |
| R-factor | 0.208 |
| Rwork | 0.202 |
| R-free | 0.25990 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.595 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.690 | 3.110 |
| High resolution limit [Å] | 2.990 | 2.990 |
| Number of reflections | 18575 | |
| <I/σ(I)> | 10.15 | |
| Completeness [%] | 99.4 | |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 25.5% polyethylene glycol 4000 and 0.17 M ammonium sulfate |
