6DC4
RSV-neutralizing human antibody AM22
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X6A |
Synchrotron site | NSLS |
Beamline | X6A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-03-25 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 1.000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 64.240, 75.220, 109.010 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.720 - 1.700 |
R-factor | 0.1812 |
Rwork | 0.180 |
R-free | 0.20490 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3lmj 3qeg |
RMSD bond length | 0.006 |
RMSD bond angle | 0.836 |
Data reduction software | MOSFLM (7.2.2) |
Data scaling software | Aimless (0.7.1) |
Phasing software | PHASER (2.8.0) |
Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 32.720 | 1.730 |
High resolution limit [Å] | 1.700 | 1.700 |
Number of reflections | 58786 | 20172 |
<I/σ(I)> | 17.4 | 2.3 |
Completeness [%] | 100.0 | 100 |
Redundancy | 7.1 | 6.5 |
CC(1/2) | 0.999 | 0.714 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.02 M 1,6-Hexanediol, 0.02 M 1-Butanol, 0.02 M 1,2-Propanediol (racemic), 0.02 M 2-Propanol, 0.02 M 1,4-Butanediol, 0.02 M 1,3-Propanediol, 0.1 M MES/imidazole pH 6.5, 10% PEG 8000, 20% ethylene glycol |