6D8A
RsAgo Ternary Complex with guide RNA and Target DNA Containing A-A Bulge Within the Seed Segment of the Target Strand
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-04-05 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 68.411, 118.985, 118.818 |
| Unit cell angles | 90.00, 95.51, 90.00 |
Refinement procedure
| Resolution | 42.665 - 2.251 |
| Rwork | 0.198 |
| R-free | 0.23900 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.330 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.090 | 0.650 |
| Number of reflections | 88612 | |
| <I/σ(I)> | 11.8 | 2.4 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 3.7 | 3.6 |
| CC(1/2) | 0.989 | 0.754 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 40% MPD, 30 mM magnesium acetate, 50 mM sodium cacodylate pH 5.7 |
