6D7T
Cryo-EM structure of human TRPV6-Y467A in complex with 2-Aminoethoxydiphenyl borate (2-APB)
EM specimen
| Vitrification | Yes |
EM Data Collection
| Microscope | FEI TECNAI 20 |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 200 |
| Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 4.44 |
| Software | RELION,MotionCorr2,PHENIX |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
