6D7M

Crystal structure of the W184R/W231R Importin alpha mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-03-07
Detector	DECTRIS PILATUS 200K
Wavelength(s)	1.514
Spacegroup name	P 21 21 21
Unit cell lengths	77.813, 90.162, 98.394
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.907 - 2.187
R-factor	0.193
Rwork	0.191
R-free	0.22220
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5e6q
RMSD bond length	0.006
RMSD bond angle	0.808
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.240
High resolution limit [Å]	2.187	2.200
Rmeas		0.843
Rpim	0.043	0.336
Number of reflections	35979	1705
<I/σ(I)>	9.2	2.2
Completeness [%]	99.1	96.5
Redundancy	6.3	6
CC(1/2)		0.814

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	0.1M Bis-Tris-Propane, 1.0M Sodium Succinate