6D3A

Structure of human ARH3 D314E bound to ADP-ribose and magnesium

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-11-16
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97
Spacegroup name	P 1
Unit cell lengths	44.901, 72.229, 115.906
Unit cell angles	83.01, 85.73, 72.43

Refinement procedure

Resolution	68.483 - 1.600
R-factor	0.168559378874
Rwork	0.167
R-free	0.19847
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.939
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	phenix.refine (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	68.480	1.618
High resolution limit [Å]	1.600	1.600
Number of reflections	173752
<I/σ(I)>	17.6
Completeness [%]	95.7
Redundancy	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	22% PEG4000, 0.1 M sodium acetate pH 4.5, and 0.1 M MgSO4