6D2P
Crystal structure of IOMA-class CLK31 Fab from an HIV-1 naive donor in complex with a germline-targeting gp120 engineered outer domain eOD-GT8 at 2.6 A
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 293 |
| Detector technology | PIXEL |
| Collection date | 2017-10-28 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.03320 |
| Spacegroup name | P 21 21 2 |
| 格子定数 [Å] | 134.300, 66.636, 76.518 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 38.259 - 2.600 |
| R因子 | 0.216 |
| Rwork | 0.213 |
| R-free | 0.26760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ies |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.003 |
| 結合角の平均二乗偏差(RMSD) [度] | 0.618 |
| Data reduction software | HKL-2000 (705b) |
| Data scaling software | HKL-2000 (705b) |
| Phasing software | PHASER (2.7.17) |
| Refinement software | PHENIX (1.9_1692) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 38.260 | 2.720 |
| 分解能 [Å] (高) | 2.600 | 2.600 |
| 独立反射数 | 21985 | 2165 |
| <I/σ(I)> | 7 | |
| 完全性 [%] | 99.9 | 100 |
| 冗長性 | 10.9 | 10.8 |
| CC(1/2) | 0.920 | 0.310 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | pH 5.0 of 20% w/v PEG6000, 0.1M citric acid (pH 4.0) |
