6D0Y
X-ray Crystal Structure of PGC-1beta C-terminus bound to the CBP80-CBP20 Cap Binding Complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-03-22 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.9553 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 78.106, 111.897, 124.795 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.053 - 2.676 |
R-factor | 0.185 |
Rwork | 0.181 |
R-free | 0.24010 |
RMSD bond length | 0.004 |
RMSD bond angle | 0.552 |
Data reduction software | XDS |
Data scaling software | Aimless (0.5.4) |
Phasing software | PHENIX |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 39.050 | 39.050 | 2.800 |
High resolution limit [Å] | 2.670 | 8.870 | 2.670 |
Rmerge | 0.211 | 0.067 | 1.188 |
Rmeas | 0.234 | 0.074 | 1.338 |
Rpim | 0.100 | 0.032 | 0.602 |
Total number of observations | 167278 | 4903 | 17492 |
Number of reflections | 30950 | 961 | 3554 |
<I/σ(I)> | 5.9 | 10.5 | 2.1 |
Completeness [%] | 97.9 | 98.5 | 86.2 |
Redundancy | 5.4 | 5.1 | 4.9 |
CC(1/2) | 0.980 | 0.991 | 0.349 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 0.2 M magnesium chloride, 0.1 M Tris (pH 7.0), 10% [w/v] polyethylene glycol 8000 |