6CZO
The KNL1-PP1 Holoenzyme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-11-14 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.03 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 138.049, 138.049, 118.423 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.125 - 2.950 |
| R-factor | 0.172 |
| Rwork | 0.169 |
| R-free | 0.21300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4mov |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.615 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.125 | 3.000 |
| High resolution limit [Å] | 2.946 | 2.950 |
| Rmerge | 0.089 | 0.665 |
| Number of reflections | 24812 | 1214 |
| <I/σ(I)> | 29.2 | 4.4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.9 | 8.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 10% PEG 6000, 0.1 M HEPES, VAPOR DIFFUSION, TEMPERATURE 277K |
