6CYT
HIV-1 TAR loop in complex with Tat:AFF4:P-TEFb
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-10-11 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.11583 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 148.580, 148.580, 104.630 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 128.674 - 3.500 |
| R-factor | 0.251 |
| Rwork | 0.247 |
| R-free | 0.28290 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5l1z |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 140.000 | 3.625 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Number of reflections | 17145 | 1690 |
| <I/σ(I)> | 11.1 | 0.63 |
| Completeness [%] | 99.5 | 96.1 |
| Redundancy | 37.6 | 16.3 |
| CC(1/2) | 0.999 | 0.441 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 291 | 50 mM Tris 8.5, 0.2M Ammonium Acetate, 6 mM MgCl2, 8% PEG 4K |
