6CXZ
RNA octamer containing 2'-F, 4'-Calpha-Me U.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-06-29 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.9184 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 30.460, 30.460, 81.889 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.439 - 1.500 |
R-factor | 0.1877 |
Rwork | 0.186 |
R-free | 0.21540 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5vr4 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.13_2992) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 25.000 | 25.000 | 1.530 |
High resolution limit [Å] | 1.500 | 4.070 | 1.500 |
Rmerge | 0.101 | 0.052 | 0.988 |
Rmeas | 0.104 | 0.053 | 1.040 |
Rpim | 0.022 | 0.012 | 0.313 |
Total number of observations | 145275 | ||
Number of reflections | 6684 | 417 | 328 |
<I/σ(I)> | 8.7 | ||
Completeness [%] | 99.8 | 100 | 99.7 |
Redundancy | 21.6 | 21.1 | 9.5 |
CC(1/2) | 0.999 | 0.808 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 1.0 mM RNA, 40 mM Na cacodylate, 20 mM magnesium chloride, 20 mM cobalt(3+) hexamine chloride, 40% MPD |