6CWU
Protein Tyrosine Phosphatase 1B F135Y mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.3 |
Synchrotron site | ALS |
Beamline | 5.0.3 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-12-12 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.976 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 89.197, 89.197, 105.734 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 77.250 - 2.080 |
R-factor | 0.25645 |
Rwork | 0.255 |
R-free | 0.29122 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3a5j |
RMSD bond length | 0.006 |
RMSD bond angle | 1.046 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 77.250 | 2.120 |
High resolution limit [Å] | 2.080 | 2.080 |
Rmerge | 0.101 | 2.080 |
Rpim | 0.033 | 0.655 |
Number of reflections | 29715 | |
<I/σ(I)> | 14.4 | |
Completeness [%] | 100.0 | |
Redundancy | 10.5 | |
CC(1/2) | 0.999 | 0.720 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277.15 | 100 mM HEPES, 200 mM magnesium acetate, and 14% polyethylene glycol 8000, pH 7.5 |